Journal: eLife

Article Title: UBE3A-mediated p18/LAMTOR1 ubiquitination and degradation regulate mTORC1 activity and synaptic plasticity

doi: 10.7554/eLife.37993

Figure Lengend Snippet: Antibodies, chemicals, and plasmids used in this study

Article Snippet: Antibody , anti-MP1 (rabbit monoclonal) , Cell Signaling Technology , 8168; RRID: AB_10949501 , (1:1000).

Techniques: Recombinant

Journal: eLife

Article Title: UBE3A-mediated p18/LAMTOR1 ubiquitination and degradation regulate mTORC1 activity and synaptic plasticity

doi: 10.7554/eLife.37993

Figure Lengend Snippet:

Article Snippet: Antibody , anti-MP1 (rabbit monoclonal) , Cell Signaling Technology , 8168; RRID: AB_10949501 , (1:1000).

Techniques: Control, shRNA, Virus, Sequencing, Recombinant, Mutagenesis, Ubiquitin Proteomics, Software

Journal: The EMBO Journal

Article Title: Micropeptide hSPAR regulates glutamine levels and suppresses mammary tumor growth via a TRIM21-P27KIP1-mTOR axis

doi: 10.1038/s44318-024-00359-z

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit anti-MAPKSP1 , Proteintech, USA , Cat #11937-1-AP.

Techniques: Recombinant, Sequencing, Modification, Membrane, Lysis, Transfection, Magnetic Beads, Software, Cytometry, In Vitro, cDNA Synthesis, Plasmid Preparation, Isolation, Mutagenesis, Silver Staining

Journal: Journal of Cellular and Molecular Medicine

Article Title: Circ_0075829 facilitates the progression of pancreatic carcinoma by sponging miR‐1287‐5p and activating LAMTOR3 signalling

doi: 10.1111/jcmm.16089

Figure Lengend Snippet: LAMTOR3 expression was stimulated by circ_0075829 through sponging miR‐1287‐5p. A, Six downstream targets (BSCL2, RFX7, LAMTOR3, LONP2, ISY1 and RAB43) of miR‐1287‐5p predicted by bioinformatics tools (TargetScan, miRDB, miRPathDB and miRTarBase) as shown by the Venn diagram. B, LAMTOR3 expression in paratumour and tumour tissues from 38 PC patients by RT‐PCR, normalized by β‐actin expression. C, LAMTOR3 expression negatively associated with miR‐1287‐5p expression in PC samples. D, LAMTOR3 expression level in SW1990 and BxPC‐3 cells after transfection with Sh‐circ_0075829 or co‐transfection with miR‐1287‐5p inhibitor. E, Mutated binding sites on LAMTOR3 for miR‐1287‐5p, and luciferase activity of LAMTOR3‐reporter in 293T cells co‐transfected with miR‐1287‐5p mimics compared with those transfected with NC. F, Western blot analysis for the protein expression of LAMTOR3, p‐ERK, ERK, p‐AKT, AKT in circ_0075829‐knockdown SW1990 and BxPC‐3 cells or cells co‐transfected with miR‐1287‐5p inhibitor or NC. Data are from at least three independent experiments and expressed as mean ± SD (* P < .05)

Article Snippet: The membranes were blocked with 5% BSA (Beyotime) and incubated (diluted 1:1000 in buffer) with the following antibodies: LAMTOR3 (CST, #5436), cyclin D (CST, #2978), E‐cadherin (Proteintech, 20874‐1‐AP), N‐cadherin (Proteintech, 22018‐1‐AP), ERK (CST, #6495), p‐ERK (CST, #4370), AKT (CST, #4691), p‐AKT (CST, #4060) and β‐actin (1:2000, CST, #5174) at 4°C overnight.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Transfection, Cotransfection, Binding Assay, Luciferase, Activity Assay, Western Blot, Knockdown

Journal: Molecular Medicine Reports

Article Title: Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts

doi: 10.3892/mmr.2017.6437

Figure Lengend Snippet: PI3K, Akt, mTOR gene primer sequences and amplification fragment length of the product.

Article Snippet: 9474S) and rabbit anti-human mTOR monoclonal antibody (dilution: 1:500; catalog no. 8665T) were from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Amplification, Sequencing

Journal: Molecular Medicine Reports

Article Title: Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts

doi: 10.3892/mmr.2017.6437

Figure Lengend Snippet: mRNA relative expression quantities of TGF-β1, PI3K, Akt, and mTOR in the two groups.

Article Snippet: 9474S) and rabbit anti-human mTOR monoclonal antibody (dilution: 1:500; catalog no. 8665T) were from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts

doi: 10.3892/mmr.2017.6437

Figure Lengend Snippet: The protein relative expression quantities of TGF-β1, PI3K, Akt, and mTOR in the two groups.

Article Snippet: 9474S) and rabbit anti-human mTOR monoclonal antibody (dilution: 1:500; catalog no. 8665T) were from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts

doi: 10.3892/mmr.2017.6437

Figure Lengend Snippet: Protein expression of electrophoresis of transforming growth factor-β1 (TGF-β1), phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), mammalian target of rapamycin (mTOR), β-actin of the two groups.

Article Snippet: 9474S) and rabbit anti-human mTOR monoclonal antibody (dilution: 1:500; catalog no. 8665T) were from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing, Electrophoresis